5KYN
Structure of Sec23 and TANGO1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-13 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 133.454, 65.880, 229.871 |
| Unit cell angles | 90.00, 97.39, 90.00 |
Refinement procedure
| Resolution | 47.940 - 2.552 |
| R-factor | 0.2018 |
| Rwork | 0.200 |
| R-free | 0.24640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3egd |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.563 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.550 |
| Number of reflections | 57059 |
| <I/σ(I)> | 18.1 |
| Completeness [%] | 99.8 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 100mM HEPES PH 7.5, 6% (v/v ) isopropanol and 300 mM sodium citrate |
