5KYL
Crystal structure of Staphylococcal nuclease variant Delta+PHS T62K/V66E pH 7 at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2015-08-13 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.062, 60.498, 38.300 |
| Unit cell angles | 90.00, 94.28, 90.00 |
Refinement procedure
| Resolution | 38.190 - 1.850 |
| R-factor | 0.1797 |
| Rwork | 0.177 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.854 |
| Data reduction software | CrysalisPro (1.171.36.32) |
| Data scaling software | CrysalisPro (1.171.36.32) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.020 | 0.105 |
| Number of reflections | 12150 | |
| <I/σ(I)> | 50.97 | 8.03 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.1 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 30% MPD, 25 mM potassium phosphate, calcium chloride, pdTp |
