5KYD
Crystal structure of USP7 catalytic domain [V302K] mutant in complex with ubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-05-11 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.050, 80.470, 86.560 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.160 - 1.620 |
R-factor | 0.1756 |
Rwork | 0.174 |
R-free | 0.21330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nb8 1ubq |
RMSD bond length | 0.006 |
RMSD bond angle | 0.866 |
Data reduction software | MOSFLM (7.1.1) |
Data scaling software | Aimless (0.5.1) |
Phasing software | PHASER (1.10.1) |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 27.160 | 27.160 | 1.640 |
High resolution limit [Å] | 1.620 | 8.700 | 1.625 |
Rmerge | 0.080 | 0.044 | 0.997 |
Number of reflections | 55856 | ||
<I/σ(I)> | 10.3 | ||
Completeness [%] | 99.4 | 97 | 93.8 |
Redundancy | 5.8 | 6.1 | 5.7 |
CC(1/2) | 0.998 | 0.997 | 0.562 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | 0.2M Ammonium fluoride, 20% PEG3350 |