5KY7

mouse POFUT1 in complex with O-glucosylated EGF(+) and GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-11
Detector	RAYONIX MX-300
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	51.890, 66.440, 108.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.801 - 1.600
R-factor	0.1964
Rwork	0.195
R-free	0.21570
RMSD bond length	0.005
RMSD bond angle	1.128
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.064	0.805
Number of reflections	49185
<I/σ(I)>	13.5	2.2
Completeness [%]	98.0	97
Redundancy	5.7	5.9
CC(1/2)	0.998	0.765

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	20% PEG2000 MME, 50 mM Tris pH 8.5