5KY2

mouse POFUT1 in complex with O-glucosylated mouse Factor VII EGF1 and GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-01
Detector	RAYONIX MX-300
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	51.965, 67.138, 109.357
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.935 - 1.470
R-factor	0.1543
Rwork	0.153
R-free	0.17490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.283
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.520
High resolution limit [Å]	1.470	1.470
Rmerge	0.123	1.524
Number of reflections	66097
<I/σ(I)>	8.3	1
Completeness [%]	100.0	100
Redundancy	7.2	6.5
CC(1/2)	0.994	0.466

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	20% PEG2000 MME, 50 mM Tris pH 8.5