5KXQ
mouse POFUT1 in complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-11 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.140, 251.820, 56.080 |
| Unit cell angles | 90.00, 95.81, 90.00 |
Refinement procedure
| Resolution | 46.499 - 1.900 |
| R-factor | 0.163 |
| Rwork | 0.162 |
| R-free | 0.19070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.122 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_2341) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.036 | 0.421 |
| Number of reflections | 114238 | |
| <I/σ(I)> | 14.2 | 1.9 |
| Completeness [%] | 94.0 | 90 |
| Redundancy | 2.2 | 2.1 |
| CC(1/2) | 0.999 | 0.746 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 1.5M malonic acid, pH 6.0 |
