5KWN
The WD domain ofArabidopsis thaliana E3 Ubiquitin Ligase COP1 in complex with peptide from HY5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-22 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97920 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 47.990, 55.176, 131.618 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.775 - 1.420 |
R-factor | 0.1603 |
Rwork | 0.160 |
R-free | 0.17360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5igo |
RMSD bond length | 0.009 |
RMSD bond angle | 1.069 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.775 | 1.471 |
High resolution limit [Å] | 1.420 | 1.420 |
Rmerge | 0.153 | |
Number of reflections | 66069 | |
<I/σ(I)> | 22.97 | 6.09 |
Completeness [%] | 99.0 | 100 |
Redundancy | 2.9 | 2.9 |
CC(1/2) | 0.992 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | Sodium Chloride 0.3M Hepes 7.2 0.1M PEG 3350 25% |