5KWL
expanded poliovirus in complex with VHH 10E
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI POLARA 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.8 |
| Software | e2boxer.py,RELION,GeFrealign and Frealign,REFMAC,CTFFIND3,Coot |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
