5KVK
Crystal structure of the Competence-Damaged Protein (CinA) Superfamily Protein KP700603 from Klebsiella pneumoniae 700603
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2016-07-13 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 38.460, 38.460, 178.238 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.115 - 1.660 |
R-factor | 0.1634 |
Rwork | 0.161 |
R-free | 0.20660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5kol |
RMSD bond length | 0.014 |
RMSD bond angle | 1.473 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.115 | 1.690 |
High resolution limit [Å] | 1.660 | 1.660 |
Rmerge | 0.062 | 0.595 |
Number of reflections | 16307 | |
<I/σ(I)> | 52.37 | 3.08 |
Completeness [%] | 96.6 | 80.4 |
Redundancy | 7.1 | 4.7 |
CC(1/2) | 0.680 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | 0.1 M sodium acetate pH 4.5, 30% PEG 300, 0.3 M NDSB 195 |