5KVK

Crystal structure of the Competence-Damaged Protein (CinA) Superfamily Protein KP700603 from Klebsiella pneumoniae 700603

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-07-13
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	38.460, 38.460, 178.238
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.115 - 1.660
R-factor	0.1634
Rwork	0.161
R-free	0.20660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5kol
RMSD bond length	0.014
RMSD bond angle	1.473
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.115	1.690
High resolution limit [Å]	1.660	1.660
Rmerge	0.062	0.595
Number of reflections	16307
<I/σ(I)>	52.37	3.08
Completeness [%]	96.6	80.4
Redundancy	7.1	4.7
CC(1/2)		0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	298	0.1 M sodium acetate pH 4.5, 30% PEG 300, 0.3 M NDSB 195