5KVJ

Crystal structure of the 16-mer doublestranded RNA. Northeast Structural Genomics Consortium (NESG) target RNA1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-05-05
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	H 3
Unit cell lengths	43.299, 43.299, 124.441
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	21.649 - 2.261
R-factor	0.1958
Rwork	0.194
R-free	0.23340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3nd3
RMSD bond length	0.010
RMSD bond angle	1.341
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.300
High resolution limit [Å]	2.260	2.260
Rmerge	0.028	0.334
Number of reflections	3824
<I/σ(I)>	37.2	3.5
Completeness [%]	93.6	97.7
Redundancy	2	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microbatch under paraffin oil	9	291	2.4 M Ammonium sulfate, 0.1 M BICINE, pH 9.0, dsRNA buffer: 0.025M NH4OAc, pH 5.5, 0.275 M NaCl, 0.005 M CaCl2, 0.001 M EDTA, 0.001 M TCEP, 0.025 M Arg, and 0.02% NaN3