5KVF

Zika specific antibody, ZV-64, bound to ZIKA envelope DIII

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2016-06-15
Detector	RDI CMOS_8M
Wavelength(s)	1.00004
Spacegroup name	P 21 21 21
Unit cell lengths	59.680, 92.160, 96.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.743 - 1.400
R-factor	0.1554
Rwork	0.154
R-free	0.18650
RMSD bond length	0.005
RMSD bond angle	0.848
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.743		1.440
High resolution limit [Å]	1.400	6.260	1.400
Rmerge	0.082	0.028	1.462
Number of reflections	105012
<I/σ(I)>	19.68	68.13	1.75
Completeness [%]	99.9	95	100
Redundancy	10.4
CC(1/2)	0.999	0.999	0.582

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1 M sodium acetate and 22% PEG4000