5KVD
Zika specific antibody, ZV-2, bound to ZIKA envelope DIII
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2016-05-28 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.00004 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.020, 113.440, 132.970 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.650 - 1.650 |
R-factor | 0.1916 |
Rwork | 0.190 |
R-free | 0.22120 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.752 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 33.650 | 1.690 | |
High resolution limit [Å] | 1.650 | 7.380 | 1.650 |
Rmerge | 0.059 | 0.021 | 0.989 |
Number of reflections | 71401 | ||
<I/σ(I)> | 18.76 | 61.04 | 2.04 |
Completeness [%] | 98.9 | 94.6 | 100 |
Redundancy | 6.7 | ||
CC(1/2) | 0.999 | 0.999 | 0.710 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M MES pH6.5, 0.6 M NaCl and 20.6% PEG 4000 |