5KUK

Crystal Structure of Inward Rectifier Kir2.2 K62W Mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	90
Detector technology	PIXEL
Collection date	2015-06-21
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	I 4
Unit cell lengths	82.695, 82.695, 182.441
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	91.390 - 2.000
R-factor	0.20038
Rwork	0.199
R-free	0.22667
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3spi
RMSD bond length	0.005
RMSD bond angle	1.025
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP (5.8.0048)
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	91.390	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.076	0.740
Number of reflections	40670
<I/σ(I)>	14.32	1.7
Completeness [%]	99.6	100
Redundancy	2.9	2.8
CC(1/2)		0.675

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293.15	tri-sodium citrate, NaCl, PEG 400