5KU2
expanded poliovirus in complex with VHH 7A
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI POLARA 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 5.3 |
| Software | e2boxer.py,FREALIGN,Relion 1.3,GeFrealign,CTFFIND3,REFMAC 5.8.0124,SerialEM,Coot,RELION |
| Symmetry | I |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
