5KTZ
expanded poliovirus in complex with VHH 12B
EM specimen
Vitrification | Yes |
EM Data Collection
Microscope | FEI POLARA 300 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 4.3 |
Software | e2boxer.py,FREALIGN,Relion 1.3,GeFrealign,REFMAC 5.8.0124,SerialEM,CTFFIND3,Coot,RELION |
Symmetry | I |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |