5KTJ

Crystal structure of Pistol, a class of self-cleaving ribozyme

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	77.15
Detector technology	PIXEL
Collection date	2016-06-16
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.8445, 1.0163,1.8450
Spacegroup name	I 41 2 2
Unit cell lengths	85.190, 85.190, 256.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	81.270 - 2.970
R-factor	0.19834
Rwork	0.196
R-free	0.25719
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	2.212
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELXDE
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.270
High resolution limit [Å]	2.971	2.971
Number of reflections	10205
<I/σ(I)>	11.87
Completeness [%]	99.8
Redundancy	17.36

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298.15	6% MPD, 0.02 M magnesium chloride, 0.02 M cobalt hexammine, 0.04 M sodium cacodylate