5KTG

Crystal structure of mouse Bak BH3-in-groove homodimer (GFP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-11
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	171.641, 171.641, 98.194
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.548 - 2.802
R-factor	0.2455
Rwork	0.244
R-free	0.27460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bdu
RMSD bond length	0.019
RMSD bond angle	2.155
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	40122
<I/σ(I)>	13.1
Completeness [%]	97.4
Redundancy	14.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	7-12% PEG3350, 20% MPD, 100 mM Tris, pH 7.0-8.5, 0.5% CHAPS