5KTE
Crystal structure of Deinococcus radiodurans MntH, an Nramp-family transition metal transporter
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 177 |
Detector technology | CCD |
Collection date | 2011-02-24 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.139 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 113.131, 132.077, 220.995 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.471 - 3.941 |
R-factor | 0.2722 |
Rwork | 0.268 |
R-free | 0.31310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4WGW |
RMSD bond length | 0.003 |
RMSD bond angle | 0.740 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.471 | 4.080 |
High resolution limit [Å] | 3.941 | 3.940 |
Rmerge | 0.171 | |
Number of reflections | 11791 | |
<I/σ(I)> | 6 | 0.61 |
Completeness [%] | 95.0 | 96 |
Redundancy | 16.6 | 11.4 |
CC(1/2) | 0.990 | 0.189 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.5 | 277 | 0.1 M sodium acetate pH 4.5, 0.05 M magnesium acetate, 24% PEG400 and 0.4-1 % beta-octylglucoside |
2 | VAPOR DIFFUSION | 4.5 | 277 | 0.1 M sodium acetate pH 4.5, 0.05 M magnesium acetate, 24% PEG400 and 0.4-1 % beta-octylglucoside |
3 | VAPOR DIFFUSION | 4.5 | 277 | 0.1 M sodium acetate pH 4.5, 0.05 M magnesium acetate, 24% PEG400 and 0.4-1 % beta-octylglucoside |