5KT4
Teranry complex of human DNA polymerase iota R96G inserting dCMPNPP opposite template G in the presence of Mg2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-19 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 98.119, 98.119, 202.720 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.059 - 2.780 |
R-factor | 0.2187 |
Rwork | 0.217 |
R-free | 0.24950 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.653 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.830 |
High resolution limit [Å] | 2.780 | 7.540 | 2.780 |
Rmerge | 0.100 | 0.030 | 0.922 |
Number of reflections | 15324 | ||
<I/σ(I)> | 7 | ||
Completeness [%] | 100.0 | 99.9 | 100 |
Redundancy | 17.7 | 17.6 | 17.4 |
CC(1/2) | 0.999 | 0.954 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277.15 | 18% PEG MME 5000, 0.1M MES buffer, 0.3M ammonium sulfate |