5KT0
Dihydrodipicolinate reductase from the industrial and evolutionarily important cyanobacteria Anabaena variabilis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 72.728, 89.361, 95.917 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 2.899 - 2.830 |
| R-factor | 0.18629 |
| Rwork | 0.183 |
| R-free | 0.24346 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.503 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 55.500 |
| High resolution limit [Å] | 2.820 |
| Number of reflections | 7762 |
| <I/σ(I)> | 25.76 |
| Completeness [%] | 99.7 |
| Redundancy | 14.18 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 281 | 21% PEG3350, 0.2 M lithium sulphate, 0.1 M bis-tris chloride pH 5.5. |
