5KSY
hMiro1 C-domain GDP Complex P41212 Crystal Form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-01-29 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.978720 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 51.900, 51.900, 151.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.765 - 2.482 |
| R-factor | 0.2708 |
| Rwork | 0.266 |
| R-free | 0.31490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c0l |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.715 |
| Data reduction software | MOSFLM (7.1.0) |
| Data scaling software | Aimless (0.3.8) |
| Phasing software | PHENIX (2.5.6) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.900 | |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.132 | |
| Number of reflections | 7933 | |
| <I/σ(I)> | 15.1 | |
| Completeness [%] | 100.0 | |
| Redundancy | 25.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | 12 mg/mL protein, 0.1 M Tris, pH 8.5, 16% w/v PEG10000, GDP observed in the structure was carried through from the expression system during purification |
