5KST
Stationary phase Survival protein E (SurE) from Xylella fastidiosa- XfSurE-TSAmp (Tetramer Smaller - crystallization with 3'AMP).
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE W01B-MX2 |
Synchrotron site | LNLS |
Beamline | W01B-MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-15 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.428 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 157.001, 78.209, 82.158 |
Unit cell angles | 90.00, 96.74, 90.00 |
Refinement procedure
Resolution | 77.957 - 2.759 |
R-factor | 0.1991 |
Rwork | 0.197 |
R-free | 0.24830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ty2 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.820 |
Data reduction software | XDS (30/03/2013) |
Data scaling software | XSCALE (30/03/2013) |
Phasing software | PHASER (2.3.0) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 77.957 | 2.840 |
High resolution limit [Å] | 2.760 | 2.760 |
Rmerge | 0.574 | |
Number of reflections | 24993 | |
<I/σ(I)> | 16.58 | 3.4 |
Completeness [%] | 97.4 | 92.1 |
Redundancy | 5.47 | 5.13 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | 0.1 M bis-tris propane pH 7.6, 0.14 M Sodium iodide, 20% (W/V) PEG 3350, 5 mM dithiotheitol and 0.1 manganese II chloride. |