5KSP

hMiro1 C-domain GDP Complex C2221 Crystal Form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-02
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.978560
Spacegroup name	C 2 2 21
Unit cell lengths	66.070, 72.640, 151.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.877 - 2.162
R-factor	0.1954
Rwork	0.193
R-free	0.24120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4c0l
RMSD bond length	0.003
RMSD bond angle	0.616
Data reduction software	MOSFLM (7.1.0)
Data scaling software	Aimless (0.3.8)
Phasing software	PHENIX (2.5.6)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.900
High resolution limit [Å]	2.160	2.160
Rmerge	0.148
Number of reflections	19991
<I/σ(I)>	14.8
Completeness [%]	100.0
Redundancy	14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	294	15 mg/mL protein, 6 mM magnesium chloride, 0.1 M Tris, pH 8.5, 12% w/v PEG4000, GDP observed in the structure was carried through from the expression system during purification