5KSA
Bel602-DQ8.5-glia-gamma1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.548, 98.831, 80.175 |
| Unit cell angles | 90.00, 95.69, 90.00 |
Refinement procedure
| Resolution | 45.750 - 2.000 |
| R-factor | 0.171 |
| Rwork | 0.169 |
| R-free | 0.20600 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.070 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 45.750 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 65534 |
| <I/σ(I)> | 12.52 |
| Completeness [%] | 100.0 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.1 M Ca-Acetate, 21 % PEG3350, Tris pH 8.0 |
