5KRQ
Renalase in complex with NADPH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.445, 71.046, 74.807 |
| Unit cell angles | 90.00, 107.79, 90.00 |
Refinement procedure
| Resolution | 46.020 - 2.086 |
| R-factor | 0.1591 |
| Rwork | 0.157 |
| R-free | 0.20280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kkj |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.225 |
| Data reduction software | HKL-2000 (712) |
| Data scaling software | SCALEPACK (712) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | PHENIX ((dev_2328: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.020 | 2.161 |
| High resolution limit [Å] | 2.086 | 2.086 |
| Rmerge | 0.071 | 0.357 |
| Number of reflections | 37538 | |
| <I/σ(I)> | 11.21 | 2.57 |
| Completeness [%] | 98.0 | 96 |
| Redundancy | 3.2 | 2.9 |
| CC(1/2) | 0.999 | 0.888 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 2 M sodium formate, 100 mM sodium acetate pH 4.6 |
