5KRG

RNA 15mer duplex binding with PZG monomer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	99
Detector technology	CCD
Collection date	2016-01-26
Detector	MAR CCD 165 mm
Wavelength(s)	0.97
Spacegroup name	P 3
Unit cell lengths	44.046, 44.046, 84.516
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.258 - 1.600
R-factor	0.20711
Rwork	0.206
R-free	0.23384
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nfo
RMSD bond length	0.024
RMSD bond angle	2.656
Data reduction software	HKL-2000 (2000)
Data scaling software	HKL-2000 (2000)
Phasing software	PHASER (2.5.6)
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.110	0.270
Number of reflections	21914
<I/σ(I)>	11.9	2.18
Completeness [%]	95.6	71.3
Redundancy	4.5	1.6
CC(1/2)	0.966	0.941

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	0.01 M Magnesium chloride hexahydrate, 0.05 M HEPES sodium pH 7.0, 4.0 M Lithium chloride