5KRG
RNA 15mer duplex binding with PZG monomer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 99 |
| Detector technology | CCD |
| Collection date | 2016-01-26 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 3 |
| Unit cell lengths | 44.046, 44.046, 84.516 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.258 - 1.600 |
| R-factor | 0.20711 |
| Rwork | 0.206 |
| R-free | 0.23384 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nfo |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.656 |
| Data reduction software | HKL-2000 (2000) |
| Data scaling software | HKL-2000 (2000) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.110 | 0.270 |
| Number of reflections | 21914 | |
| <I/σ(I)> | 11.9 | 2.18 |
| Completeness [%] | 95.6 | 71.3 |
| Redundancy | 4.5 | 1.6 |
| CC(1/2) | 0.966 | 0.941 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.01 M Magnesium chloride hexahydrate, 0.05 M HEPES sodium pH 7.0, 4.0 M Lithium chloride |
