5KR0

Protease E35D-APV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-03-10
Detector	RIGAKU
Wavelength(s)	1.5418
Spacegroup name	P 2 21 21
Unit cell lengths	45.650, 57.740, 86.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	17.735 - 1.800
R-factor	0.1808
Rwork	0.177
R-free	0.21300
RMSD bond length	0.007
RMSD bond angle	1.061
Data reduction software	iMOSFLM (7.1.1)
Data scaling software	Aimless
Phasing software	PHASER (2.1)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	18.800
High resolution limit [Å]	1.800
Number of reflections	21795
<I/σ(I)>	10.7
Completeness [%]	99.9
Redundancy	6.37

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		300	sodium chloride