5KQX

Protease E35D-SQV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-10-30
Detector	RIGAKU
Wavelength(s)	1.5418
Spacegroup name	P 2 21 21
Unit cell lengths	45.960, 58.420, 85.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.153 - 2.400
R-factor	0.1768
Rwork	0.170
R-free	0.23610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.102
Data reduction software	iMOSFLM (7.1.1)
Data scaling software	Aimless
Phasing software	PHASER (2.1)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	25.700
High resolution limit [Å]	2.400
Number of reflections	9541
<I/σ(I)>	8
Completeness [%]	99.9
Redundancy	6.55

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		300	sodium chloride