5KPY
Structure of a 5-hydroxytryptophan aptamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-09-15 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 127.553, 26.594, 63.367 |
| Unit cell angles | 90.00, 106.32, 90.00 |
Refinement procedure
| Resolution | 18.333 - 2.000 |
| R-factor | 0.2206 |
| Rwork | 0.216 |
| R-free | 0.25810 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.308 |
| Data reduction software | CrystalClear |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.950 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.084 | 0.191 |
| Number of reflections | 13725 | |
| <I/σ(I)> | 11.2 | 5.4 |
| Completeness [%] | 96.2 | 73.8 |
| Redundancy | 4.34 | 3.65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 303 | 8-14% MPD, 40 mM sodium cacodylate, pH 5.5, 4 mM MgCl2, 12 mM NaCl, 80 mM KCl, 4-9 mM cobalt hexamine |
