5KPI

Mouse native PGP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-24
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	100.607, 119.535, 383.887
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.138 - 4.010
R-factor	0.2599
Rwork	0.258
R-free	0.30260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m1m
RMSD bond length	0.007
RMSD bond angle	1.023
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX ((dev_2443: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.405	50.000	4.120
High resolution limit [Å]	4.000	9.140	4.000
Rmerge	0.072	0.036	0.855
Rmeas	0.080
Rpim	0.035
Total number of observations	187287
Number of reflections	37665
<I/σ(I)>	7.6
Completeness [%]	95.3	94.6	82
Redundancy	5	4.9	4
CC(1/2)		0.999	0.452

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	50 mM Glycine9.0 100 mM NaCl, 22-24% PEG400