5KPI
Mouse native PGP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-24 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 100.607, 119.535, 383.887 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.138 - 4.010 |
R-factor | 0.2599 |
Rwork | 0.258 |
R-free | 0.30260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4m1m |
RMSD bond length | 0.007 |
RMSD bond angle | 1.023 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX ((dev_2443: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 35.405 | 50.000 | 4.120 |
High resolution limit [Å] | 4.000 | 9.140 | 4.000 |
Rmerge | 0.072 | 0.036 | 0.855 |
Rmeas | 0.080 | ||
Rpim | 0.035 | ||
Total number of observations | 187287 | ||
Number of reflections | 37665 | ||
<I/σ(I)> | 7.6 | ||
Completeness [%] | 95.3 | 94.6 | 82 |
Redundancy | 5 | 4.9 | 4 |
CC(1/2) | 0.999 | 0.452 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 50 mM Glycine9.0 100 mM NaCl, 22-24% PEG400 |