5KP6
Crystal Structure of the Curacin Biosynthetic Pathway HMG Synthase in Complex with Apo Donor-ACP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 101.312, 101.312, 104.593 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 87.740 - 2.050 |
| R-factor | 0.1572 |
| Rwork | 0.155 |
| R-free | 0.19320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5kp5 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.476 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 87.740 |
| High resolution limit [Å] | 2.050 |
| Rmerge | 0.117 |
| Number of reflections | 39403 |
| <I/σ(I)> | 19.1 |
| Completeness [%] | 100.0 |
| Redundancy | 19.5 |
| CC(1/2) | 0.990 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 6% PEG 8000, 60 mM (NH4)2SO4, 1X MMT pH 6.5 |
