5KOR
Arabidopsis thaliana fucosyltransferase 1 (FUT1) in complex with GDP and a xylo-oligossacharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.87257 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.975, 85.806, 150.723 |
| Unit cell angles | 90.00, 96.19, 90.00 |
Refinement procedure
| Resolution | 39.678 - 2.200 |
| R-factor | 0.1807 |
| Rwork | 0.179 |
| R-free | 0.21490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5kop |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.097 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.280 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 113138 | |
| <I/σ(I)> | 8.3 | 2 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 4.4 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | HEPES, PEG 8000, sodium chloride |
