5KOR
Arabidopsis thaliana fucosyltransferase 1 (FUT1) in complex with GDP and a xylo-oligossacharide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.87257 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 87.975, 85.806, 150.723 |
Unit cell angles | 90.00, 96.19, 90.00 |
Refinement procedure
Resolution | 39.678 - 2.200 |
R-factor | 0.1807 |
Rwork | 0.179 |
R-free | 0.21490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5kop |
RMSD bond length | 0.010 |
RMSD bond angle | 1.097 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.280 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 113138 | |
<I/σ(I)> | 8.3 | 2 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 4.4 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | HEPES, PEG 8000, sodium chloride |