5KOL
Crystal structure of the competence-damaged protein (CinA) superfamily protein ECK1530/EC0983 from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-17 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.978560 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 76.690, 89.150, 245.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.885 - 1.910 |
| R-factor | 0.2088 |
| Rwork | 0.206 |
| R-free | 0.25110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a9s |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.837 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.885 | 1.960 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.069 | 0.855 |
| Number of reflections | 65002 | |
| <I/σ(I)> | 16.1 | 2.2 |
| Completeness [%] | 99.2 | 94.3 |
| Redundancy | 6.1 | 6 |
| CC(1/2) | 0.999 | 0.838 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 1.5 M ammonium chloride, 0.1 M sodium acetate pH 4.6 |
