5KOL

Crystal structure of the competence-damaged protein (CinA) superfamily protein ECK1530/EC0983 from Escherichia coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-17
Detector	RAYONIX MX-300
Wavelength(s)	0.978560
Spacegroup name	C 2 2 21
Unit cell lengths	76.690, 89.150, 245.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.885 - 1.910
R-factor	0.2088
Rwork	0.206
R-free	0.25110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2a9s
RMSD bond length	0.007
RMSD bond angle	0.837
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.885	1.960
High resolution limit [Å]	1.910	1.910
Rmerge	0.069	0.855
Number of reflections	65002
<I/σ(I)>	16.1	2.2
Completeness [%]	99.2	94.3
Redundancy	6.1	6
CC(1/2)	0.999	0.838

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	298	1.5 M ammonium chloride, 0.1 M sodium acetate pH 4.6