5KO7
Crystal structure of haliscomenobacter hydrossis iodotyrosine deiodinase (IYD) bound to FMN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.127 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 92.185, 110.881, 43.288 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.443 - 2.248 |
R-factor | 0.2176 |
Rwork | 0.216 |
R-free | 0.25390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gb5 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.707 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 36.960 |
High resolution limit [Å] | 2.248 |
Rmerge | 0.070 |
Number of reflections | 21635 |
<I/σ(I)> | 15.2 |
Completeness [%] | 98.9 |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 80 mM Tris-HCl pH 8.5, 100 mM MgCl2, 24% (w/v) PEG 4000 and 20% glycerol |