5KO0
Human Islet Amyloid Polypeptide Segment 15-FLVHSSNNFGA-25 Determined by MicroED
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ELECTRON MICROSCOPE |
Source details | TECNAI F20 TEM |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2014-11-04 |
Detector | TVIPS F416 CMOS CAMERA |
Wavelength(s) | 0.0251 |
Spacegroup name | P 1 |
Unit cell lengths | 11.680, 18.180, 19.930 |
Unit cell angles | 62.84, 88.90, 87.60 |
Refinement procedure
Resolution | 9.749 - 1.400 |
R-factor | 0.2287 |
Rwork | 0.225 |
R-free | 0.25900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.216 |
Data scaling software | XSCALE |
Phasing software | PHASER (2.5.6) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 17.733 | 17.733 | 1.440 |
High resolution limit [Å] | 1.400 | 6.260 | 1.400 |
Rmerge | 0.193 | 0.140 | 0.505 |
Rmeas | 0.215 | 0.158 | 0.648 |
Total number of observations | 9014 | ||
Number of reflections | 2180 | 23 | 84 |
<I/σ(I)> | 4.33 | 8.3 | 1.1 |
Completeness [%] | 75.0 | 71.9 | 35.3 |
CC(1/2) | 0.985 | 0.984 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 2 uL 20 mg/mL FLVHSSNNFGA + 1 uL 35% MPD, 0.35 M sodium thiocyanate against a reservoir of 35% MPD, 0.35 M sodium thiocyanate. Crystals appeared within several hours. |