5KNI

Crystal Structure of the wild-type SAM domain of human Tankyrase-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-28
Detector	ADSC QUANTUM 315
Wavelength(s)	1.03319
Spacegroup name	P 1 21 1
Unit cell lengths	87.322, 55.101, 92.441
Unit cell angles	90.00, 115.88, 90.00

Refinement procedure

Resolution	30.000 - 2.500
R-factor	0.17042
Rwork	0.168
R-free	0.21218
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3h8m
RMSD bond length	0.013
RMSD bond angle	1.603
Data reduction software	XDS
Data scaling software	XDS
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.500
Number of reflections	30719
<I/σ(I)>	10.7
Completeness [%]	98.4
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2 M magnesium formate