5KN5
TGFalpha/Epiregulin complex with neutralizing antibody LY3016859
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 193 |
Detector technology | CCD |
Collection date | 2011-03-18 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.97931 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.249, 164.346, 64.972 |
Unit cell angles | 90.00, 104.21, 90.00 |
Refinement procedure
Resolution | 24.995 - 2.800 |
Rwork | 0.206 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.16) |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 24.995 | 24.995 | 2.950 |
High resolution limit [Å] | 2.800 | 8.850 | 2.800 |
Rmerge | 0.028 | 0.612 | |
Rmeas | 0.149 | ||
Rpim | 0.077 | ||
Total number of observations | 117046 | ||
Number of reflections | 31162 | ||
<I/σ(I)> | 9.6 | 21 | 1.3 |
Completeness [%] | 98.5 | 91.8 | 99.9 |
Redundancy | 3.8 | 3.6 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294 | 100mM MES pH 6, 14% PEG 3350, 200mM Sodium Sulfate |