5KN2
Native bovine skeletal calsequestrin, high-Ca2+ form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2016-05-28 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 130.363, 169.194, 155.477 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.947 - 2.601 |
R-factor | 0.2066 |
Rwork | 0.205 |
R-free | 0.24110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5kn1 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.843 |
Data reduction software | HKL-2000 (v712) |
Data scaling software | HKL-2000 (v712) |
Phasing software | PHENIX (1.10_2155) |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.957 | 2.674 |
High resolution limit [Å] | 2.601 | 2.601 |
Rmerge | 0.063 | 1.416 |
Number of reflections | 52807 | |
<I/σ(I)> | 13.25 | 2.17 |
Completeness [%] | 100.0 | 97 |
Redundancy | 6.9 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 0.1 M HEPES, 27.5 % 2-methyl-2,4-pentanediol, 0.2 M NaCl |