5KN2
Native bovine skeletal calsequestrin, high-Ca2+ form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2016-05-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 130.363, 169.194, 155.477 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.947 - 2.601 |
| R-factor | 0.2066 |
| Rwork | 0.205 |
| R-free | 0.24110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5kn1 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.843 |
| Data reduction software | HKL-2000 (v712) |
| Data scaling software | HKL-2000 (v712) |
| Phasing software | PHENIX (1.10_2155) |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.957 | 2.674 |
| High resolution limit [Å] | 2.601 | 2.601 |
| Rmerge | 0.063 | 1.416 |
| Number of reflections | 52807 | |
| <I/σ(I)> | 13.25 | 2.17 |
| Completeness [%] | 100.0 | 97 |
| Redundancy | 6.9 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 0.1 M HEPES, 27.5 % 2-methyl-2,4-pentanediol, 0.2 M NaCl |
