5KMY
Crystal structure of tryptophan synthase subunit alpha from Legionella pneumophila str. Paris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.707, 69.654, 75.075 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.538 - 1.908 |
| R-factor | 0.1775 |
| Rwork | 0.176 |
| R-free | 0.20670 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.897 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (dev_1888) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.538 | 1.930 |
| High resolution limit [Å] | 1.908 | 1.908 |
| Rmerge | 0.062 | 0.490 |
| Number of reflections | 31628 | |
| <I/σ(I)> | 20 | |
| Completeness [%] | 99.7 | 96.9 |
| Redundancy | 4.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | sodium chloride (0.2M) bis-tris ph 6.5 (0.1M) peg 3350 (25%) |
