5KMX

Structure of Trypanosoma congolense Insect Stage Antigen

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	75.810, 87.651, 98.891
Unit cell angles	90.00, 113.27, 90.00

Refinement procedure

Resolution	39.472 - 2.452
R-factor	0.2592
Rwork	0.258
R-free	0.28890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5klh
RMSD bond length	0.003
RMSD bond angle	0.763
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.472	2.540
High resolution limit [Å]	2.450	2.452
Rmerge	0.044	0.517
Number of reflections	42706
<I/σ(I)>	17.8	1.2
Completeness [%]	97.6	98.34
Redundancy	3.4	3.5
CC(1/2)	0.960	0.821

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	291	20% PEG 3350 with 0.2 M (NH4)2SO4