5KMX
Structure of Trypanosoma congolense Insect Stage Antigen
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-11-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 75.810, 87.651, 98.891 |
Unit cell angles | 90.00, 113.27, 90.00 |
Refinement procedure
Resolution | 39.472 - 2.452 |
R-factor | 0.2592 |
Rwork | 0.258 |
R-free | 0.28890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5klh |
RMSD bond length | 0.003 |
RMSD bond angle | 0.763 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.472 | 2.540 |
High resolution limit [Å] | 2.450 | 2.452 |
Rmerge | 0.044 | 0.517 |
Number of reflections | 42706 | |
<I/σ(I)> | 17.8 | 1.2 |
Completeness [%] | 97.6 | 98.34 |
Redundancy | 3.4 | 3.5 |
CC(1/2) | 0.960 | 0.821 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 291 | 20% PEG 3350 with 0.2 M (NH4)2SO4 |