5KMT
CTX-M9 mutant L48A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-03 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 |
| Unit cell lengths | 41.788, 41.788, 232.401 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.220 - 1.700 |
| R-factor | 0.19543 |
| Rwork | 0.194 |
| R-free | 0.21607 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.588 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 77.470 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 47330 |
| <I/σ(I)> | 26.8 |
| Completeness [%] | 94.6 |
| Redundancy | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 294 | 1.2 M potassium phosphate |
