5KM9
Human Histidine Triad Nucleotide Binding Protein 2 (hHint2) adenosine complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.753, 74.771, 77.863 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.931 - 1.450 |
| R-factor | 0.1601 |
| Rwork | 0.160 |
| R-free | 0.16870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4inc |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.235 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 53.931 | 53.931 | 1.455 |
| High resolution limit [Å] | 1.450 | 6.726 | 1.450 |
| Rmerge | 0.043 | 0.021 | 0.360 |
| Rmeas | 0.047 | ||
| Rpim | 0.019 | ||
| Total number of observations | 250634 | ||
| Number of reflections | 39791 | ||
| <I/σ(I)> | 22 | ||
| Completeness [%] | 99.8 | 100 | 100 |
| Redundancy | 6.3 | 5.2 | 6.4 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100 mM MES, 38% PEG 8000 |
