5KLH
Crystal Structure of Trypanosoma brucei Procyclic Specific Surface Antigen-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 31.820, 57.810, 61.240 |
| Unit cell angles | 73.55, 76.43, 89.98 |
Refinement procedure
| Resolution | 47.122 - 1.646 |
| R-factor | 0.1788 |
| Rwork | 0.177 |
| R-free | 0.21410 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.068 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 56.950 |
| High resolution limit [Å] | 1.646 |
| Rmerge | 0.066 |
| Number of reflections | 45400 |
| <I/σ(I)> | 10.3 |
| Completeness [%] | 92.3 |
| Redundancy | 3.8 |
| CC(1/2) | 0.995 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.2 M (NH4)2SO4, 0.1 M Bis-tris pH 6.5, and 25% PEG 3350 |
