5KLB
Crystal structure of the CavAb voltage-gated calcium channel(wild-type, 2.7A)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 124.900, 125.700, 191.485 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.932 - 2.700 |
R-factor | 0.2212 |
Rwork | 0.219 |
R-free | 0.26000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ms2 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.613 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1839) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.850 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.114 | 0.984 |
Number of reflections | 770488 | |
<I/σ(I)> | 13.4 | 2.4 |
Completeness [%] | 92.5 | 97.8 |
Redundancy | 10.1 | 9.8 |
CC(1/2) | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | CHAPSO:DMPC BICELLES,0.1M Na-citrate,pH5.0,2M Ammonium Sulfate |