5KL8
Crystal structure of the Pumilio-Nos-CyclinB RNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-26 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.992 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 135.102, 135.102, 220.402 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.001 - 4.000 |
| R-factor | 0.2863 |
| Rwork | 0.283 |
| R-free | 0.31190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.508 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 4.070 |
| High resolution limit [Å] | 4.000 | 10.820 | 4.000 |
| Rmerge | 0.143 | 0.101 | 0.779 |
| Number of reflections | 10740 | ||
| <I/σ(I)> | 7.4 | ||
| Completeness [%] | 99.3 | 92.4 | 100 |
| Redundancy | 8.9 | 5.8 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.9 M ammonium sulfate, 0.1 M MES, pH 5.6 |
