5KL1

Crystal structure of the Pumilio-Nos-hunchback RNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-24
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 65 2 2
Unit cell lengths	137.007, 137.007, 221.401
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.325 - 3.701
R-factor	0.2679
Rwork	0.264
R-free	0.30020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3h3d 3alr
RMSD bond length	0.003
RMSD bond angle	0.605
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.760
High resolution limit [Å]	3.700	10.020	3.700
Rmerge	0.128	0.074	0.747
Rmeas	0.135
Rpim	0.042
Total number of observations	154091
Number of reflections	13652
<I/σ(I)>	5.2
Completeness [%]	99.9	99.1	100
Redundancy	11.3	9.1	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.1 M ammonium sulfate, 0.1 M MES, pH 5.6