5KL0

Crystal Structure of Phosphoglucomutase from Xanthomonas citri citri complexed with Glucose-1,6-biphosphate

Replaces:  5BMR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-10-24
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	43.980, 54.788, 173.495
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.613 - 1.853
R-factor	0.1475
Rwork	0.145
R-free	0.18510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5bmn
RMSD bond length	0.008
RMSD bond angle	0.893
Data reduction software	d*TREK
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.613	19.613	1.960
High resolution limit [Å]	1.850	5.370	1.850
Rmerge	0.020	0.017	0.041
Number of reflections	35635
<I/σ(I)>	45.06	62.88	22.89
Completeness [%]	97.4	95.4	89.5
Redundancy	3.71
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	12.5 % PEG 1000, 12.5 % PEG 3350, 12.5 % MPD, 30 mM MgCl2, 30 mM CaCl2, 100 mM MES/Imidazole pH 6.5