5KKV
Metal-mediated coiled-coil GCN4-p2L peptide assembly crystal
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2015-08-01 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.033 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 38.328, 38.328, 46.165 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.950 - 1.900 |
R-factor | 0.227 |
Rwork | 0.226 |
R-free | 0.24050 |
Structure solution method | SAD |
RMSD bond length | 0.001 |
RMSD bond angle | 0.390 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 3324 | |
<I/σ(I)> | 23.7 | |
Completeness [%] | 99.7 | |
Redundancy | 10.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 7.1 | 295 | 20mM MOPS buffer, pH 7.1 |