5KKC
l-lactate dehydrogenase from rabbit muscle with the inhibitor 6DHNAD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-20 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.602, 126.085, 84.237 |
| Unit cell angles | 90.00, 99.94, 90.00 |
Refinement procedure
| Resolution | 39.408 - 1.859 |
| R-factor | 0.1704 |
| Rwork | 0.170 |
| R-free | 0.21120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4i9h |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.145 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX (1.10_2155) |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.410 | 1.890 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.066 | 0.574 |
| Number of reflections | 108172 | |
| <I/σ(I)> | 11.8 | 2 |
| Completeness [%] | 98.7 | 93.2 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1 M Tris, pH 7.5, 0.08 M sodium acetate, 14% PEG 8,000 |
