5KJH
Crystal structure of an active polycomb repressive complex 2 in the stimulated state
Replaces: 5CH1Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2015-03-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.554, 74.612, 128.508 |
| Unit cell angles | 90.00, 107.70, 90.00 |
Refinement procedure
| Resolution | 45.840 - 2.270 |
| R-factor | 0.1702 |
| Rwork | 0.168 |
| R-free | 0.21280 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.060 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmerge | 0.582 | |
| Number of reflections | 61840 | |
| <I/σ(I)> | 28.14 | 1.81 |
| Completeness [%] | 97.6 | 81.6 |
| Redundancy | 6.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 15% PEG4000, 175mM ammonium citrate, pH 7.0 | |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 15% PEG4000, 175mM ammonium citrate, pH 7.0 |
